Tesmin^em2Qsh

CRISPR/Cas9 technology generated a 76 bp deletion in exon 9 resulting in a C-terminal truncated protein (p.Ser445Argfs*3). This truncation mutation eliminates TESMINs interaction with LIN9 and consequently leads to cytoplasmic retention of TESMIN in pachytene spermatocytes. Western blot analysis confirmed the presence of truncated protein in the testes of homozygous mutant males. (J:324057)