Tnfaip3^em1Stgr

Using an sgRNA (targeting GGGATATCTGTAACACTCC) and an ssODN template (CACTGTCCTTCAGGGTCACCAGGGGTACAAAGTGCTGGCTGTCATAGCCTAGGACGATGGGATATCTGTAATACTCCTGAGCAGGCCAGTGAAGAGGCAGATAAATCCCACCCACTTTCAAAGGAGCAAAATTGGAACCAGATTCCAAAC) with CRISPR/Cas9 technology, cysteine codon 243 (TGT) was changed to tyrosine (TAT) (c.728G>A, p.C243Y). (J:283545)