Mice were generated by inserting a 2A sequence, YFP, 2A sequence, and the N-terminal fragment of tTA (aa 1-205) fused to the N terminal 102 amino acids of the DnaE split intein sequence from Nostoc punctiforme (Npu), FLAG tag, and Frt-flanked neomycin resistance cassette immediately in front of the stop codon of Sftpc coding sequence by homologous recombination. The Frt-flanked neomycin resistance cassette was removed by crossing with hACTB-Flpe deleter mice. (J:323339)
Basic Information
(C57BL/6 x 129S4/SvJae)F1
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count