Adgrl2^tm1.3Sud

A targeting vector was designed to introduce a loxP site upstream of the first coding exon (exon 2). A segment carrying an F1 FRT site, FRT site, 1 kb of the mouse engrailed splice acceptor, 5 kb of Adgrl2 cDNA fused to mVenus green fluorescent protein, a polyA signal, FRT site, PGK-neomycin resistance cassette, tandem FRT and F1 FRT sites, and a loxP site were placed in intron 2. Cre and Flp-mediated recombination in the germline resulted in the removal of exon1, the cDNA/Venus gene fusion and the neomycin selection cassette. (J:246190)