The targeting construct inserted a loxP site upstream of exon 4 and an FRT- and loxP-flanked neomycin resistance cassette downstream of exon 5. Flp-mediated recombination removed the selection cassette and left exons 4 and 5 floxed. (J:273223)
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The targeting construct inserted a loxP site upstream of exon 4 and an FRT- and loxP-flanked neomycin resistance cassette downstream of exon 5. Flp-mediated recombination removed the selection cassette and left exons 4 and 5 floxed. (J:273223)