CRISPR-targeting inserted a loxP site upstream of exon 2 and an FRT-flanked neomycin resistance cassette with a 3' loxP site downstream of exon 2. Flp-mediated recombination removed the selection cassette and left exon 2 floxed. (J:317466)
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CRISPR-targeting inserted a loxP site upstream of exon 2 and an FRT-flanked neomycin resistance cassette with a 3' loxP site downstream of exon 2. Flp-mediated recombination removed the selection cassette and left exon 2 floxed. (J:317466)