Casp8^em2Gne

Zygotes carrying the Casp8em1Gne allele (p.D225A and p.D218A and Casp8tm1.1Gne allele p.D387A) were targeted with sgRNAs (targeting AGAGATGAGCCTCAAAATGG) and an ssODN template (AAACAGGAACACCAATGCAAAGAGAGAGGTAGGAGACACACCTACCCGTGACTCACTAGCTTGTTCTCTTGGCGAGGCACAGAGCTCCGCCATCTTCAGGCTCATCTCTGCAAGCAACCAAAAAAACGTCACACTTAGGACAACCTAAGGACC) template using CRISPR/Cas9 technology to change aspartic acid codon 212 (GAC) in exon 8 to alanine (GCC) (p.D212A). (J:281456)