Parg^{tm2c(KOMP)Mbp}

The L1L2_Bact_P cassette was inserted at position 31929646 of Chromosome 14 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 31932826. FLP excised by microinjecting 100 ng/ul of FLP mRNA (Miltenyi BIotec Inc. Cat# 130-106-769) into the cytoplasm of IVF-derived zygotes. This resulted in the removal of the neo selection cassette and lacZ reporter leaving behind the floxed critical exon(s). (J:322048)