The targeting construct inserted an IRES-mCherry reporter gene in the 3'UTR of Nkx2-1. Puromycin selection gene was removed in embryonic stem cells using Flipase (FlpO). (J:322048)
Basic Information
(129X1/SvJ x 129S1/Sv)F1-Kitl+
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count