The targeting construct inserted an IRES-mCherry reporter gene in the 3'UTR of Nkx2-1. Puromycin selection gene was removed in embryonic stem cells using Flipase (FlpO). (J:322048)
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The targeting construct inserted an IRES-mCherry reporter gene in the 3'UTR of Nkx2-1. Puromycin selection gene was removed in embryonic stem cells using Flipase (FlpO). (J:322048)