Mafb^tm1.2Mardu

Histidine codon 131 (CAC) in the single exon was changed to glutamine (CAG) (p.H131Q) and an FRT site flanked neomycin resistance gene cassette and a loxP site were inserted upstream and second loxP site downstream. The mutation is associated with orofacial clefts (OFCs) in humans. The neo cassette was removed through subsequent flp-mediated recombination and the entire single-exon gene deleted through cre-mediated recombination. (J:311539)