A loxP site was inserted 5' of the second exon and a second loxP was inserted 3' of the fourth exon. An frt-flanked neomycin selection cassette was removed via flp-mediated recombination leaving exons 2-4 floxed. (J:322052)
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A loxP site was inserted 5' of the second exon and a second loxP was inserted 3' of the fourth exon. An frt-flanked neomycin selection cassette was removed via flp-mediated recombination leaving exons 2-4 floxed. (J:322052)