Gt(ROSA)26Sor^{tm1.1(CAG-YFP/DROSHA*E1147K)Gess}

Using ES cells containing the RMCE-ready Gt(ROSA)26Sortm1Rkuhn allele, the original cassette was exchanged with a vector containing the following elements: an attB site, the CAG promoter, a loxP site flanked puromycin resistance gene cassette, a YFP gene in-frame with a human DROSHA cDNA with a p.E1147K mutation and C-terminal FLAG tag sequences, and a second attB site. The mutated Drosha will only be expressed after cre-mediated deletion of the puro cassette. The mutation is associated with Wilms Tumor and critically affects the metal-binding Drosha needs for its cleaving activity. (J:321428)