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A BAC containing the Gt(ROSA)26Sor gene (BAC_ESr2-445b1_sfi_1) was modified by replacing part of intron 1 with a loxP site flanked neomycin resistance gene cassette, the Cas9 gene, sequence for P2A self-cleaving peptide sequence and the GFP reporter gene. The BAC replaced the endogenous locus through homologous recombination. The neo cassette was removed through subsequent cre-mediated recombination, leaving an allele expressing Cas9 and GFP driven by the Gt(ROSA)26Sor promoter. (J:317226)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Gene Expression
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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References Literature
Title
PMID
Journal
Year
IF
