Mecp2^em2Gfng

CRISPR/cas9 genome editing used two guides sgRNA-49229 (5'-gattgcatactttgaaaagg-3') and sgRNA-49791 (5'-ccacccttggtgagaaaagc-3') to target the start and end of the humanization region (exon 4) as well as a single strand DNA (ssDNA) donor template that spans the two cut sides. The homology arm length for the ssDNA donor is 300 nt. The insertion is comprised of a 561 bp humanized MECP2 region, which corresponds to amino acid (aa) positions 145-331 aa in the human MeCP2 locus (Note: brain enriched isoform MeCP2-E1 is 498 aa in length). (J:101977)