Ikzf3^em3Itan

Using an sgRNA (targeting GCAGTTTAATATGACGGAGG in exon 4) and an ssODN template (CCTTGTGATCAGCGATCTCCTTTTTCCTCCTTTCTGAAGGCGAACGCCCGTTCCAGTGTAATCAGTGCGGGGCATCTTTTACTCAGAAACGTAATCTCCTCCGTCATATTAAACTGCACACGGGGGAAAAACCTTTTAAGTGTCACCTCTGCAACTACGCATGCCAAAGGAGAGATGCGCTCACGGGACACCTTAGGACA) with CRISPR/Cas9 technology resulted in the insertion of several nucleotides into leucine codon 161, creating a premature stop codon three codons downstream. (J:319672)