Exon 2 and 3 were flanked by loxP sites. An FRT-flanked puromycin selection cassette was inserted dodwnsteam of exon 3 before the second loxP site and was removed via flp-mediated recombination. (J:321295)
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Exon 2 and 3 were flanked by loxP sites. An FRT-flanked puromycin selection cassette was inserted dodwnsteam of exon 3 before the second loxP site and was removed via flp-mediated recombination. (J:321295)