CRISPR/cas9 mediated HDR using a single stranded oligo, which included homology arms, is used to insert a Bxb1 attP-GT site (48 bp) into intron 1 of the ROSA26 locus. (J:322700)
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CRISPR/cas9 mediated HDR using a single stranded oligo, which included homology arms, is used to insert a Bxb1 attP-GT site (48 bp) into intron 1 of the ROSA26 locus. (J:322700)