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EN
Targeting vector PG00061_Z_2_D09 from KOMP project was electroporated into ES cells. The targeting cassette is composed of En2 intron/splice-acceptor/exon, an IRES, the beta-galactosidase (LacZ) gene, a polyadenylation signal (pA) a loxP site, a beta-actin promoter, the neomycin-resistance gene (neo), a second pA site, second FRT site, and second loxP site. A third loxP site was inserted downstream of exon 3. In situ hybridization confirmed absence of mRNA in homozygotes. (J:320628)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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| Phenotypes |
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References Literature
Title
PMID
Journal
Year
IF
