The targeting vector inserted a loxP site upstream of the coding sequence in the first coding exon and an FRT-flanked neomycin resistance cassette with a 3 prime loxP site. Flp-mediated recombination removed the selection cassette. (J:318731)
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The targeting vector inserted a loxP site upstream of the coding sequence in the first coding exon and an FRT-flanked neomycin resistance cassette with a 3 prime loxP site. Flp-mediated recombination removed the selection cassette. (J:318731)