The targeting construct inserted four knock-in point mutations in the alpha I helix of the DNA-binding domain. A triple FLAG tag was introduced at the N-terminus. Cre-mediated recombination removed the selection cassette. (J:319847)
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The targeting construct inserted four knock-in point mutations in the alpha I helix of the DNA-binding domain. A triple FLAG tag was introduced at the N-terminus. Cre-mediated recombination removed the selection cassette. (J:319847)