An FRT flanked neo cassette and loxP site were inserted upstream of exon 2 and a loxP site was inserted downstream of exon 3 via homologous recombination. Flp-mediated recombination removed the selection cassette. (J:213769)
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An FRT flanked neo cassette and loxP site were inserted upstream of exon 2 and a loxP site was inserted downstream of exon 3 via homologous recombination. Flp-mediated recombination removed the selection cassette. (J:213769)