A loxP site was inserted upstream of exon 1 and a loxP site and FRT flanked neo cassette were inserted downstream of exon 1 via homologous recombination. Flp and cre mediated recombination removed the neo cassette and exon 1. (J:317520)
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A loxP site was inserted upstream of exon 1 and a loxP site and FRT flanked neo cassette were inserted downstream of exon 1 via homologous recombination. Flp and cre mediated recombination removed the neo cassette and exon 1. (J:317520)