CRISPR-targeting inserted a loxP site upstream of exon 4 and a rox-flanked neomycin resistance cassette with a 3 prime loxP site downstream of exon 4. Dre-mediated recombination removed the selection cassette and left exon 4 floxed. (J:312681)
查看原文 参与反馈
CRISPR-targeting inserted a loxP site upstream of exon 4 and a rox-flanked neomycin resistance cassette with a 3 prime loxP site downstream of exon 4. Dre-mediated recombination removed the selection cassette and left exon 4 floxed. (J:312681)