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A mouse sonic hedgehog cDNA lacking the sequence for the cholesterol attachment and with a p.C25S mutation (to prevent palmitoylation of the peptide) was placed under the control of the mouse paired related homeobox 1 enhancer to drive its expression in embryonic limb buds. The NonCholesterol Modified mutation involves inserting a loxP site flanked neomycin resistance gene cassette into the coding region in exon 3 upstream of the cholesterylation site, which results in a premature stop codon after glycine codon 198. The neo cassette was removed through subsequent cre-mediated recombination. (J:89228)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Gene Expression
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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References Literature
Title
PMID
Journal
Year
IF
