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The targeting vector is designed to replace the entire coding region of the gene with an enhanced green fluorescent protein (EGFP) sequence. An FRT-flanked neomycin resistance (neo) cassette with a polyadenylation signal (polyA) in reverse orientation to the gene were inserted into the 3' UTR. FLP-mediated recombination removed the FRT-flanked neo cassette. Immunoblot analysis confirmed the absence of protein expression in various tissues of homozygous mutant mice. (J:316411)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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| Phenotypes |
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References Literature
Title
PMID
Journal
Year
IF
