The targeting cassette inserted a loxP site upstream of the microRNA sequence and an FRT-flanked neomycin resistance cassette with a 3' loxP site. Cre-mediated recombination removed the selection cassette and the microRNA sequence. (J:314038)
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The targeting cassette inserted a loxP site upstream of the microRNA sequence and an FRT-flanked neomycin resistance cassette with a 3' loxP site. Cre-mediated recombination removed the selection cassette and the microRNA sequence. (J:314038)