CRISPR/cas9 genome editing is used to insert a nuclear localization signal (NLS) and FLAG tag. The single-stranded oligonucleotide donor DNA template contains the following sequence: CACTGTGAAGCAGACAGTTGATATCTGTCACTTCACTGACAAGGCATGCTGTTATTATTTTCTTTTTCTGATATTAGGAAGGCTACCAAGACTATGAGCCTGAAGCCGACTACAAGGACGACGACGACAAGTAAGAATGTCATTGCACCCAATCTCCTAAGATCTGCCGGCTGCTCTTCCATGGCGTACAAGTGCTCAGT. (J:101977)
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count