The targeting construct inserted an FRT-flanked neomycin resistance cassette with a 5' loxP site upstream of exon 1 and an additional loxP site downstream of exon 1. Cre-mediated recombination removed the selection cassette and exon 1. (J:314589)
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The targeting construct inserted an FRT-flanked neomycin resistance cassette with a 5' loxP site upstream of exon 1 and an additional loxP site downstream of exon 1. Cre-mediated recombination removed the selection cassette and exon 1. (J:314589)