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The targeting construct contains a tetracycline-responsive element (TRE; tetO) directing expression of an mCardinal (a basic far red fluorescent protein) and glycoprotein-deleted SAD-B19 rabies virus large structural protein RNA-directed RNA polymerase GTP phosphohydrolase (L_RABVS) variant B19L, separated by a picornavirus 2A element (P2A). The plasmid was then used for FLP-mediated targeted insertion into the Igs7 locus in Ai99 Flp-in embryonic stem (ES) cells derived from (129S6/SvEvTac x C57BL/6)F1 background G4 ES cells. (J:101977)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Gene Expression
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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| Phenotypes |
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References Literature
Title
PMID
Journal
Year
IF
