A loxP (L83) site is placed in intron 1 and an FRT-neomycin-FRT-loxP cassette is placed in intron 2. Flp-mediated recombination removed the FRT-flanked neo cassette leaving exon 3 floxed. (J:223363)
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A loxP (L83) site is placed in intron 1 and an FRT-neomycin-FRT-loxP cassette is placed in intron 2. Flp-mediated recombination removed the FRT-flanked neo cassette leaving exon 3 floxed. (J:223363)