A loxP site was inserted upstream of exon 4, and an FRT/loxP flanked neomycin selection cassette was inserted downstream of exon 7. The selection cassette was removed via Flp-mediated recombination leaving exons 4 to 7 floxed. (J:309372)
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A loxP site was inserted upstream of exon 4, and an FRT/loxP flanked neomycin selection cassette was inserted downstream of exon 7. The selection cassette was removed via Flp-mediated recombination leaving exons 4 to 7 floxed. (J:309372)