CRISPR-targeting introduced a loxP site upstream of exon 5 and a neomycin resistance cassette with a 3' loxP site downstream of exon 5. Cre-mediated recombination removed exon 5 and the selection cassette. (J:314970)
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CRISPR-targeting introduced a loxP site upstream of exon 5 and a neomycin resistance cassette with a 3' loxP site downstream of exon 5. Cre-mediated recombination removed exon 5 and the selection cassette. (J:314970)