A loxP site was inserted upstream of exon 4 and an FRT-flanked neomycin cassette and second loxP site were inserted downstream of exon 4. The neomycin cassette was removed via Flp-mediated recombination, leaving exon 4 floxed. (J:294414)
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A loxP site was inserted upstream of exon 4 and an FRT-flanked neomycin cassette and second loxP site were inserted downstream of exon 4. The neomycin cassette was removed via Flp-mediated recombination, leaving exon 4 floxed. (J:294414)