A loxP site was inserted upstream of exon 3 and an FRT-flanked neomycin selection cassette and second loxP site were inserted downstream of exon 3. The selection cassette was removed via Flp-mediated recombination leaving exon 3 floxed. (J:314183)
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A loxP site was inserted upstream of exon 3 and an FRT-flanked neomycin selection cassette and second loxP site were inserted downstream of exon 3. The selection cassette was removed via Flp-mediated recombination leaving exon 3 floxed. (J:314183)