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EN
Sequence for a loxP site, a OneSTrEPtag (OST) and a Gly-Ser-Gly spacer was inserted in-frame at the 5 end of the gene coding sequence and a second loxP site in inton 1. The FRT site flanked neomycin resistance gene cassette that was also inserted was removed through subsequent Flp-mediated recombination. The fusion of the OST to the protein can be used in affinity purification. (J:282407)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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| Phenotypes |
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References Literature
Title
PMID
Journal
Year
IF
