Sequence for a OneSTrEPtag (OST), two STOP codons, an IRES2, Kozak sequence, the cyan (teal) fluorescent reporter gene mTFP1, and an FRT site flanked neomycin resistance gene cassette, was inserted in-frame at the 3 end of the coding sequence. The neo cassette was removed through subsequent Flp-mediated recombination. (J:282407)