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EN
Plasmids encoding a guide RNA (GGAGTTGCAGATCACGA) are designed to insert an FRT site, a neo cassette, and a CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (CAG), followed by a second FRT site, into intron 1 of the Gt(ROSA)26Sor locus. A floxed STOP cassette followed by a mouse CD63 antigen (Cd63) sequence fused to an emerald green fluorescent protein (emGFP) was inserted downstream of the FRT flanked CAG. (J:292635)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Gene Expression
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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| Phenotypes |
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References Literature
Title
PMID
Journal
Year
IF
