The targeting construct introduced a loxP site upstream of exon 2 and an FRT-flanked puromycin resistance cassette with a 3 loxP site downstream of exon 2. Flp-mediated recombination removed the selection cassette and left exon 2 floxed. (J:312557)
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count