Tbx5^{tm1.1(cre/ERT2)Bbr}

A cDNA for Cre/ERT2, followed by an IRES element, a Kozak consensus sequence, and a 2X FLAG epitope sequence was inserted in-frame into exon 2 of the gene, upstream of the endogenous translation start site. At 40bp downstream of exon 2, cloning sites (NotI and AflII) were added and a PGK-EM7-Neo-polyA cassette, flanked by Frt sites. The neo cassette was removed by crossing with Flpe expressing mice. (J:232545)