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CRISPR/cas9 mediated recombination using a repair RNA with sequence 5'-AGGTGCCAGGAAATCAGACCTCTACTACCCTCAAGCTGTCCCCCTATGTCCACTACACaTTTgcGGTCACTGCCATTAACAAATATGGTCCTGGAGAACCCAGCCCTGTCTCTGAGACTG-3' (mutated nucleotides in small letters) created a p.R687A substitution, a restriction site for screening (AciI) and a silent mutation for a degenerated PAM sequence. This mutation disrupts the MBP cleavage site preventing the production of the L1-70 and L1-80 fragments. However, the full length protein is expressed. (J:308569)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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| Phenotypes |
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References Literature
Title
PMID
Journal
Year
IF
