Cacna1s^tm1.1Ics

A mutation was engineered in exon 13 to change asparagine codon 617 (AAC) to an aspartic acid codon (GAC)(p.N617D) and a loxP site, an FRT site flanked protamine-cre cassette, a neomycin resistance gene cassette and a second loxP site were inserted in opposite transcriptional orientation into intron 12. The floxed cre plus neo cassette pair was self-excised in male germline through the protamine promotor-driven expression of Cre. The mutation in this allele creates a non-Ca2+-conducting form of the encoded peptide. (J:252980)