Edil3^em1Gha

Aspartic acid codon 98 (GAC) was targeted for mutation to a glutamic acid codon (GAG)(p.D98E) with an sgRNA (UAUCGAGGAGACACAUUCAUGUUUUAGAGCUAGAAAUAGCAAGUUAAAAUAAGGCUAGUCCGUUAUCAACUUGAAAAAGUGGCACCGAGUCGGUGCUUUUUU), an ssODN template (TTTCAGGTCCCTGCATCCCTAACCCATGCCATAACGGAGGAACCTGTGAGATAAGCGAAGCCTATCGAGGAGAGACGTTTATAGGCTATGTTTGTAAATGTCCTCGGGGATTTAATGGGATTCACTGTCAGCACAGTAAGTTATTT) and a crRNA (targeting TATCGAGGAGACACATTCA) using CRISPR/Cas9 technology. The mutation renders the encoded peptide incapable of interacting with integrins. (J:299690)