Cd200^{tm1c(KOMP)Wtsi}

The L1L2_Bact_P cassette was inserted at position 45214682 of Chromosome 16 upstream of the critical exons 3 and 4 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exons 3 and 4 at position 45218142. The critical exons 3 and 4 are thus flanked by loxP sites. Flp-mediated recombination removed the lacZ and neomycin resistance gene creating a "conditional ready" (floxed) allele. (J:310826)