Sympk^{tm1c(EUCOMM)Wtsi}

The L1L2_gt0 cassette was inserted at position 18763987 of Chromosome 7 upstream of the critical exons 4 and 5 (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exons 4 and 5 at position 18764906. The critical exons 4 and 5 are thus flanked by loxP sites. Flp-mediated recombination removed the lacZ/neomycin sequence and created a "conditional ready" (floxed) allele. (J:255302)