Exon 5 was targeted with a gRNA (targeting GCCTGTAATACCACAGG) and an ssODN (CTAGCCATGACCTCTTCTCTTGTCCCCTAGGAGAGCTGATGATGAGCCTCCTGGGCTATTACAGGCCTGAGCACTTACAGGGAGGTCGCAGCCCCAGCATGCATGAGG) to change codon 659 from tryptophan (TGG) to glycine (GGC) (p.W659G). (J:309976)