The targeting construct inserted an FRT-flanked neomycin-STOP cassette and tetracycline response element upstream of the translation initiation site. Flp-mediated recombination removed the FRT-flanked neomycin-STOP cassette. (J:254432)
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The targeting construct inserted an FRT-flanked neomycin-STOP cassette and tetracycline response element upstream of the translation initiation site. Flp-mediated recombination removed the FRT-flanked neomycin-STOP cassette. (J:254432)