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CRISPR/cas9 endonuclease-mediated genome editing is used to insert 5 amino acid substitutions. The amino acids 30, 31, 34 in exon 2 were changed from asparagine to glutamic acid (N30D), asparagine to lysine (N31K), and glutamine to aspartic acid (Q34D) respectively, amino acid 82 in exon 3 was changed from serine to methionine (S82M), and the amino acid 353 in exon 9 was changed from histidine to lysine (H353K). The mutations are designed to completely humanize the mouse Ace2 coding sequence in a region important for binding to the SARS-CoV-2 spike protein. (J:298750)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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| Phenotypes |
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References Literature
Title
PMID
Journal
Year
IF
