Exons 4-6 were replaced with a hygromycin resistance gene flanked by FRT5 sites via homologous recombination. Flp-mediated recombination removed the hygromycin selection cassette. (J:286482, J:301535)
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Exons 4-6 were replaced with a hygromycin resistance gene flanked by FRT5 sites via homologous recombination. Flp-mediated recombination removed the hygromycin selection cassette. (J:286482, J:301535)