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Introns 1 and 2 were targeted with two sgRNAs (targeting GCTCAGGAAGCAGCCGCTTG and TAAATCCTATCTCCGGTCCC) and tracrRNAs and crRNA using CRISPR/Cas9 technology, resulting in a 2172 bp deletion that includes exon 2. Immunoblots confirmed the absence of peptide expression from this allele. (J:306305)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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| Phenotypes |
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References Literature
Title
PMID
Journal
Year
IF
