Using CRISPR-Cas9 and oligo-mediated homology directed repair, two heterologous attachment sites, Bxb1 attP-GT and Bxb1 attP-GA, were inserted into intron 1-2, providing cognate attachment and recombination sites for Bxb1 attB-GT and Bxb1 attB-GA, for unidirectional, single-copy donor transgenic integration mediated by Bxb1 Integrase. (J:188991, J:322700)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Gene Expression
Related Disease
Reference
C57BL/6J
Endonuclease-mediated
Insertion
--
--
--
1

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
Show/Hide columns
Phenotypes

References Literature

Title
PMID
Journal
Year
IF
No Data Found!
Wechat
Comparison
Al agent
Tutorials
Back to top